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 After 10 min of incubation at 30 °C, the mixture was applied to a Superdex 0 Increase 32/300 size exclusion chromatography column (GE Healthcare), equilibrated in complex buffer ( mM Na Briefly, plasmids encoding proteins were transiently expressed in HEK293T (ATCC CRL) cells The conditioned medium was dialysed and purified with a 5 mL HisTrap nickel column (GE Healthcare) and further polished using a Superdex 75 HiLoad 16/60 gel filtration column (GE Healthcare) BioLayer Interferometry The B1617 lineage of SARSCoV2, especially the delta strain, which is B, has contributed to the wave of infection in the Indian subcontinent Structural and serological analyses show some evidence of antibody escape, and individuals infected previously with the B1351 (beta) and P1 (gamma) variants are likely more susceptible to reinfection by the delta strain

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